The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma

Canine melanoma is one of the most important diseases in small animal medicine. Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein levels have been reported to exacerbate human tumor progression. The role of SET in canine melanoma, however, has not been understood. Here, we investigated the potential therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2 cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs. Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with OP449 and FTY720. These results demonstrated the potential therapeutic application of SET inhibitors for canine melanoma.     

Weak activity of UDP-glucuronosyltransferase toward Bisphenol analogs in mouse perinatal development

Bisphenol A (BPA) is a widely used industrial chemical that disrupts endocrine function. BPA is an endocrine disrupting chemical (EDC) that has been demonstrated to affect reproductive organ development, brain development, metabolic disease and post-natal behavior. Accordingly, Bisphenol analogs, Bisphenol F (BPF, bis (4-hydroxyphenyl) methane) and Bisphenol AF (BPAF, 4,4-hexafluoroisopropylidene) diphenol) are used as replacements for BPA. BPA is mainly metabolized by UDP-glucuronosyltransferase (UGT), UGT2B1, but this effective metabolizing system is weak in the fetus. In the present study, we demonstrated that hepatic UGT activity toward BPAF was very weak, in comparison with BPA and BPF, in the fetus, pups and dams. Conversely, hepatic UGT activity toward BPF was very weak in the fetus and newborn pups, and was increased to the same level as BPA post-partum. In conclusion, BPAF possibly tends to accumulate in the fetus, because of weak metabolism during the perinatal period, suggesting that the metabolism of individual Bisphenol analogs requires assessment to properly gauge their risks.     

Increased abundance of patatins,lipoxygenase and miraculins in a thaxtomin A-habituated potato Russet Burbank somaclone with enhanced resistance to common scab

Potato common scab caused by the actinobacterium Streptomyces scabiei is characterized by the formation of corky lesions on tubers that reduce their marketability. Management of common scab is very complex and often ineffective under various environmental conditions. Using potato varieties that are more resistant to common scab remains one of the most efficient strategies to control this disease. However, very little is known about the factors associated with resistance to common scab. Somaclone RB9 regenerated from thaxtomin A-habituated potato Russet Burbank calli produced tubers more resistant to common scab than the original variety. Comparison of the RB9 tuber proteome with that of Russet Burbank using label-free quantitative proteomic analysis revealed changes in the accumulation of defence-related proteins from the patatin and lipoxygenase (LOX) families, which are involved in the metabolism of lipids, and of two miraculins of the Kunitz-type protease inhibitors family. The implication of LOX during common scab infection was studied using synchronized minitubers developed from leaf-bud cuttings. S. scabiei infection stimulated the accumulation of LOX in both Russet Burbank and RB9 minitubers, but this accumulation was intensified in RB9 minitubers. Infection also increased LOX activity in Russet Burbank and RB9 minitubers. However, LOX activity measured in noninfected RB9 minitubers was similar to that of infected Russet Burbank minitubers, indicating endogenous activation of LOX activity in RB9 minitubers. We discuss how increased LOX abundance and activity in the somaclone RB9 may contribute to improving tuber defence against common scab.     

7株牛源A型巴氏杆菌的分离鉴定及耐药性分析

对2014年沈阳地区患有呼吸系统疾病的育肥牛场进行病原分离,得到7株分离菌,对其进行培养形态及染色观察、病理组织切片观察、动物致病性观察及16S序列分析,初步鉴定为牛多杀性巴氏杆菌(Pm)。利用Pm种特异性引物及荚膜血清特异性引物进行PCR扩增,均得到目的基因条带。对分离菌进行药敏试验的结果显示,5株分离菌株已对阿米卡星、新诺明产生较强耐药性;7株分离菌均对氟苯尼考、恩诺沙星、四环素及氨苄西林敏感。由此确定,分离菌株均为牛荚膜A型巴氏杆菌,且已具有一定耐药性。     

几种热带相思树种的扦插育苗试验

几种热带相思树种经大田扦插育苗试验结果表明：在广州地区以秋季进行萌条扦插育苗效果较好。以马占相思、直干大叶相思、厚荚相思萌条作插穗，用１／３火烧土加２／３黄心土作基质，蘸上自配的ＩＢＤ２＃多效生根粉，直接插入育苗容器育苗，苗床用塑料薄膜拱棚加盖遮光度为６０％的黑网以调节控制温湿度及光照，在基质和插穗不消毒、定期淋浇１‰的复合肥水的情况下，扦插成活率分别达６１．８％、８８．８％和６８．９％。其中直干大叶相思扦插易成活且受季节影响较小。     

百山祖冷杉异砧嫁接试验初报

百山祖冷杉天然更新困难，为解决这种冷杉的繁殖更新问题，采用以日本冷杉为砧木的异砧嫁接试验，初获成功。     

从澳大利亚引进的两种相思植物光合速率和蒸腾效率的初步研究

以LCA-4光合蒸腾测定系统进行大腺相思和多花相思叶片气体交换过程的测定。结果如下：（1）净光合速率日变化明显，且变化趋势大体一致，即10：00-13：00具有较高的光合速率，其它测定时间相对较低。（2）两树种净光合速率存在差异。大腺相思高于多花相思，其最大值分别为19.6(10:00),16.1(13:00)μmolm^-2s^-1，日平均值分别为11.9,9.8μmolm^-2s^-1，反映了大腺相思的生长速度较多花相思快。（3）大腺相思和多花相思蒸腾速率日变化明显，正午前后较高，13：00达到最大，日平均值多花相思略高于大腺相思，分别为3.3,3.0mmolm^-2s^-1，表明在相同的环境条件下大腺相思的耗水量低于多花相思。（4）两树种蒸腾效率的日变化趋势大体上也类似，即在9：00-11：00较高，此期间大腺相思和多花相思和多花相思的平均值分别为5.51,4.21μmolCO2/mmolH2O。日平均值大腺厢思和多花相思分别为3.84,2.88μmolCO2/mmolH2O,表明消耗等量的水分大腺相思比多花相思能固定更多的CO2量，即有更高的水分利用效率。     

旱薄山地优良肉用杏品种的引种研究

1990年－1999年，在太行山西侧旱薄山地进行了杏树的引种与选育，通过对13个杏品种树体生长量，产量及果实品质的分析，得出阳坡山地宜栽植大黄甜，华县大接杏，大杏梅，银白杏，海东杏；石灰岩山地宜发展大黄甜，大杏梅，华县大接杏;土层较厚，有施肥条件的沟块地宜发生兰州大接杏，山黄杏。     

大果沙棘不同品种果实特性比较研究

在黑龙江绥棱和内蒙磴口试验区对俄罗斯11个大果沙棘品种和蒙古1个大果沙棘品种的果实特性进行了比 较研究,结果表明:(1)黑龙江绥棱试验点百果质量在38．33-67．59 g之间,磴口试验点为32．87-63．85 g。在供 试的品种中除卡图尼在2个试验点百果质量一致外,其余品种百果质量在磴口试验点均比绥棱试验点有不同程度 的下降。引进品种除阿尔泰新闻外,其余品种的百果质量均显著低于原产地俄罗斯,反映出百果质量随纬度下降而 下降的趋势。(2)不同品种果实横径与纵径呈紧密线性相关,即随着果实纵径的增大横径随之增大。(3)果实纵 径、横径、长宽比三个指标与百果质量均达到极显著线性相关,因此可根据果实形状指标对百果质量进行预测。 (4)供试的11个大果沙棘品种果汁Vc含量在342．6-1770．3 mg·kg-1之间,与俄罗斯原产地Vc含量(470- 3300mg·kg-1)相比均有不同程度的下降。(5)供试大果沙棘品种浆果果汁中VE含量在4-29 mg·kg-1之间。 总黄酮在36-429 mg·kg-1之间,其中槲皮素、山奈酚和异鼠李素分别为21．9-310．8 mg·kg-1、1．0-9．8 mg· kg-1和13．1-133．8 mg·kg-1,很明显总黄酮主要由槲皮素和异鼠李素组成,而且二者的含量比较接近。     

刚毛柽柳 TheIF1A 基因的互作蛋白及其表达模式分析

翻译起始因子是一类翻译起始所必需的特异蛋白因子,前期研究表明柽柳翻译起始因子 (TheIF1A) 基因能对外界盐和干旱等非生物胁迫做出响应,且过表达 TheIF1A基因能提高酵母和烟草的抗旱耐盐能力。为进一步研究TheIF1A基因的抗逆机制,本研究通过酵母双杂交对柽柳翻译起始因子(TheIF1A) 基因的互作蛋白进行了筛选,共获得5个互作蛋白,分别为RNA聚合酶β II亚基 (RNA polymerase beta II subunit)、ATP合成酶CF1α亚基蛋白 (ATP synthase CF1 alpha subunit protein)、细胞色素b6/f 复合物亚基IV(cytochrome b6/f complex subunit IV)、核酮糖1,5-二磷酸羧化酶/加氧酶小亚基蛋白(ribulose 1,5-bisphosphate carboxylase/oxygenase small subunit)和组蛋白乙酰转移酶(histone acetyltransferase)。利用实时荧光定量PCR对这5个蛋白基因及 TheIF1A 基因在盐和干旱胁迫处理下的表达模式进行分析,结果表明:这些蛋白基因在盐和干旱胁迫下的表达模式与 TheIF1A基因基本一致,表明TheIF1A可能通过与这些蛋白相互作用来参与逆境胁迫应答。为进一步研究TheIF1A 基因的抗逆机理奠定了基础,有利于完善林木抗逆机制的研究,并为通过基因工程手段提高林木抗逆性提供了候选基因。